Title |
High F‑Content Perfluoropolyether-Based Nanoparticles for Targeted Detection of Breast Cancer by 19F Magnetic Resonance and Optical Imaging
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Published in |
ACS Nano, August 2018
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DOI | 10.1021/acsnano.8b03726 |
Pubmed ID | |
Authors |
Cheng Zhang, Shehzahdi Shebbrin Moonshi, Wenqian Wang, Hang Thu Ta, Yanxiao Han, Felicity Y. Han, Hui Peng, Petr Král, Barbara E. Rolfe, John Justin Gooding, Katharina Gaus, Andrew Keith Whittaker |
Abstract |
Two important challenges in the field of 19F magnetic resonance imaging (MRI) are the maintenance of high fluorine content without compromising imaging performance, and effective targeting of small particles to diseased tissue. To address these challenges, we have developed a series of perfluoropolyether (PFPE)-based hyperbranched (HBPFPE) nanoparticles with attached peptide aptamer as targeting ligands for specific in vivo detection of breast cancer with high 19F MRI sensitivity. A detailed comparison of the HBPFPE nanoparticles (NPs) with the previously reported trifluoroethyl acrylate (TFEA)-based polymers demonstrates that the mobility of fluorinated segments of the HBPFPE nanoparticles is significantly enhanced (19F T2 > 80 ms vs 31 ms), resulting in superior MR imaging sensitivity. Selective targeting was confirmed by auto- and pair correlation analysis of fluorescence microscopy data, in vitro immunofluorescence, in vivo 19F MRI, ex vivo fluorescence and 19F NMR. The results highlight the high efficiency of aptamers for targeting and the excellent sensitivity of the PFPE moieties for 19F MRI. Of relevance to in vivo applications, the PFPE-based polymers exhibit much faster clearance from the body than the previously introduced perfluorocarbon emulsions ( t1/2 ∼ 20 h vs up to months). Moreover, the aptamer-conjugated NPs show significantly higher tumor-penetration, demonstrating the potential of these imaging agents for therapeutic applications. This report of the synthesis of polymeric aptamer-conjugated PFPE-based 19F MRI CAs with high fluorine content (∼10 wt %) demonstrates that these NPs are exciting candidates for detecting diseases with high imaging sensitivity. |
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Australia | 2 | 50% |
Argentina | 1 | 25% |
United States | 1 | 25% |
Demographic breakdown
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 73 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 21 | 29% |
Researcher | 9 | 12% |
Student > Master | 7 | 10% |
Student > Doctoral Student | 6 | 8% |
Student > Bachelor | 4 | 5% |
Other | 4 | 5% |
Unknown | 22 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 19 | 26% |
Biochemistry, Genetics and Molecular Biology | 6 | 8% |
Medicine and Dentistry | 5 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 5% |
Agricultural and Biological Sciences | 3 | 4% |
Other | 13 | 18% |
Unknown | 23 | 32% |