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Mendeley readers
| Title |
Detection of Matrilysin Activity Using Polypeptide Functionalized Reduced Graphene Oxide Field-Effect Transistor Sensor
|
|---|---|
| Published in |
Analytical Chemistry, March 2016
|
| DOI | 10.1021/acs.analchem.5b04663 |
| Pubmed ID | |
| Authors |
Hu Chen, Peng Chen, Jingfeng Huang, Robert Selegård, Mark Platt, Alagappan Palaniappan, Daniel Aili, Alfred Iing Yoong Tok, Bo Liedberg |
| Abstract |
A novel approach for rapid and sensitive detection of matrilysin (MMP-7, a biomarker involved in the degradation of vari-ous macromolecules) based on polypeptide (JR2EC) functionalized reduced graphene oxide (rGO) field effect transistor (FET) is reported. MMP-7 specifically digests negatively charged JR2EC immobilized on rGO, thereby modulating the con-ductance of rGO-FET. The proposed assay enabled detection of MMP-7 at clinically relevant concentrations with a limit of detection (LOD) of 10 ng/mL (400 pM), attributed to the significant reduction of the net charge of JR2EC upon digestion by MMP-7. Quantitative detection of MMP-7 in human plasma was further demonstrated with a LOD of 40 ng/mL, illustrating the potential for the proposed methodology for tumor detection and carcinoma diagnostic (e.g. lung cancer and salivary gland cancer). Additionally, excellent specificity of the proposed assay was demonstrated using matrix metallopeptidase 1 (MMP-1), a protease of the same family. With appropriate selection and modification of polypeptides, the proposed assay could be extended for detections of other enzymes with polypeptide digestion capability. |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Unknown | 40 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 22% |
| Student > Bachelor | 6 | 15% |
| Researcher | 4 | 10% |
| Professor | 4 | 10% |
| Student > Doctoral Student | 3 | 7% |
| Other | 4 | 10% |
| Unknown | 11 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Materials Science | 9 | 22% |
| Chemistry | 5 | 12% |
| Engineering | 3 | 7% |
| Agricultural and Biological Sciences | 2 | 5% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 15 | 37% |