Title |
Plesiomonas shigelloides exports a lethal cytotoxic-enterotoxin (LCE) by membrane vesicles
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Published in |
Brazilian Journal of Infectious Diseases, October 2016
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DOI | 10.1016/j.bjid.2016.08.014 |
Pubmed ID | |
Authors |
Marilucia Santos Ludovico, Luciano Moura Martins, Juares Ednaldo Romero Bianco, Célia Guadalupe Tardelli de Jesus Andrade, Rosabel Falcon, Paulo Pinto Joazeiro, Maria Silvia Viccari Gatti, Tomomasa Yano |
Abstract |
Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure toxin presented molecular mass of 50kDa and isoelectric point (pI) around 6.9 by 2D electrophoresis. When injected intravenously, the purified cytotoxic-enterotoxin induced also severe spasms followed by sudden death of mice. Hence, we entitled it as lethal cytotoxic-enterotoxin (LCE). The presence of membrane vesicles (MVs) on cell surfaces of P. shigelloides was observed by scan electron microscopy (SEM). From these MVs the LCE toxin was extracted and confirmed by biological and serological assays. These data suggest that P. shigelloides also exports this cytotoxic-enterotoxin by membrane vesicles, a different mechanism of delivering extra cellular virulence factors, so far not described in this bacterium. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 5 | 23% |
Student > Master | 5 | 23% |
Student > Doctoral Student | 2 | 9% |
Other | 1 | 5% |
Student > Ph. D. Student | 1 | 5% |
Other | 1 | 5% |
Unknown | 7 | 32% |
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Biochemistry, Genetics and Molecular Biology | 2 | 9% |
Medicine and Dentistry | 2 | 9% |
Computer Science | 1 | 5% |
Arts and Humanities | 1 | 5% |
Other | 2 | 9% |
Unknown | 9 | 41% |