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Mendeley readers
Attention Score in Context
| Title |
qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms
|
|---|---|
| Published in |
Brazilian Journal of Infectious Diseases, November 2016
|
| DOI | 10.1016/j.bjid.2016.09.017 |
| Pubmed ID | |
| Authors |
Michelle de Campos Soriani Azevedo, Natália Mortari Ramuno, Luciana Raquel Vincenzi Fachin, Mônica Tassa, Patrícia Sammarco Rosa, Andrea de Faria Fernandes Belone, Suzana Madeira Diório, Cleverson Teixeira Soares, Gustavo Pompermaier Garlet, Ana Paula Favaro Trombone |
| Abstract |
Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary. Thus, in this study samples from 126 skin biopsies (collected from patients on all clinical forms and reactional states of leprosy) and 25 slit skin smear of leprosy patients were comparatively analyzed by qPCR (performed with primers for the RLEP region of M. leprae DNA) and routine bacilloscopy performed in histological sections or in slit skin smear. Considering clinical diagnostic as the gold standard, 84.9% of the leprosy patients were qPCR positive in skin biopsies, resulting in 84.92% sensitivity, with 84.92 and 61.22% positive (PPV) and negative (NPV) predictive values, respectively. Concerning bacilloscopy of histological sections (BI/H), the sensitivity was 80.15% and the PPV and NPV were 80.15 and 44.44%, respectively. The concordance between qPCR and BI/H was 87.30%. Regarding the slit skin smear, 84% of the samples tested positive in the qPCR. Additionally, qPCR showed 100% specificity, since all samples from different mycobacteria, from healthy individuals, and from other granulomatous diseases presented negative results. In conclusion, the qPCR technique for detection of M. leprae using RLEP primers proved to be specific and sensitive, and qPCR can be used as a complementary test to diagnose leprosy irrespective of the clinical form of disease. |
Mendeley readers
The data shown below were compiled from readership statistics for 130 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Peru | 1 | <1% |
| Unknown | 129 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 22 | 17% |
| Student > Master | 17 | 13% |
| Researcher | 13 | 10% |
| Student > Doctoral Student | 7 | 5% |
| Other | 6 | 5% |
| Other | 25 | 19% |
| Unknown | 40 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 21 | 16% |
| Medicine and Dentistry | 21 | 16% |
| Immunology and Microbiology | 12 | 9% |
| Nursing and Health Professions | 8 | 6% |
| Agricultural and Biological Sciences | 7 | 5% |
| Other | 16 | 12% |
| Unknown | 45 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2016.
All research outputs
#22,759,802
of 25,374,647 outputs
Outputs from Brazilian Journal of Infectious Diseases
#645
of 809 outputs
Outputs of similar age
#355,244
of 415,348 outputs
Outputs of similar age from Brazilian Journal of Infectious Diseases
#17
of 22 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 809 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.