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Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis

Overview of attention for article published in Brazilian Journal of Microbiology, March 2016
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Title
Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis
Published in
Brazilian Journal of Microbiology, March 2016
DOI 10.1016/j.bjm.2016.01.005
Pubmed ID
Authors

Gheniffer Fornari, Vania Aparecida Vicente, Renata Rodrigues Gomes, Marisol Dominguez Muro, Rosangela Lameira Pinheiro, Carolina Ferrari, Patricia Fernanda Herkert, Marcos Takimura, Newton Sérgio de Carvalho, Flavio Queiroz-Telles

Abstract

Vulvovaginal candidiasis affects women of reproductive age, which represents approximately 15-25% of vaginitis cases. The present study aimed to isolate and characterize yeast from the patients irrespective of the presentation of clinical symptoms. The isolates were subjected to in vitro susceptibility profile and characterization by molecular markers, which intended to assess the distribution of species. A total of 40 isolates were obtained and identified through the CHROMagar, API20aux and by ITS and D1/D2 regions sequencing of DNAr gene. Candida albicans strains were genotyped by the ABC system and the isolates were divided into two genotypic groups. The identity of the C. albicans, C. glabrata, C. guilliermondii, C. kefyr and Saccharomyces cerevisiae isolates was confirmed by the multilocus analysis. The strains of Candida, isolated from patients with complications, were found to be resistant to nystatin but sensitive to fluconazole, amphotericin B and ketoconazole, as observed by in vitro sensitivity profile. The isolates from asymptomatic patients, i.e., the colonized group, showed a dose-dependent sensitivity to the anti-fungal agents, fluconazole and amphotericin B. However, the isolates of C. albicans that belong to distinct genotypic groups showed the same in vitro susceptibility profile.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 84 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 16 19%
Student > Master 12 14%
Student > Ph. D. Student 10 12%
Researcher 5 6%
Professor 4 5%
Other 13 15%
Unknown 24 29%
Readers by discipline Count As %
Immunology and Microbiology 22 26%
Biochemistry, Genetics and Molecular Biology 12 14%
Agricultural and Biological Sciences 8 10%
Medicine and Dentistry 7 8%
Nursing and Health Professions 3 4%
Other 8 10%
Unknown 24 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2016.
All research outputs
#22,759,452
of 25,373,627 outputs
Outputs from Brazilian Journal of Microbiology
#1,047
of 1,377 outputs
Outputs of similar age
#269,380
of 312,882 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#29
of 40 outputs
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