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Mendeley readers
| Title |
Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, January 2017
|
| DOI | 10.1016/j.bjm.2016.10.021 |
| Pubmed ID | |
| Authors |
Maira de S.N. Martins, Alessandra M.M.G. de Castro, Michele dos S. Lima, Vivian da S.C. Pinto, Thaís G. da Silva, Claudia Del Fava, Claudio Regis Depes, Liria H. Okuda, Edviges M. Pituco |
| Abstract |
Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 10(1)DNA copies/μL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 10(0)DNA copy/μL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 5 | 16% |
| Student > Master | 5 | 16% |
| Researcher | 4 | 13% |
| Lecturer | 3 | 10% |
| Student > Bachelor | 3 | 10% |
| Other | 6 | 19% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 11 | 35% |
| Agricultural and Biological Sciences | 5 | 16% |
| Medicine and Dentistry | 4 | 13% |
| Economics, Econometrics and Finance | 1 | 3% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 9 | 29% |