Title |
Antimicrobial activity evaluation and comparison of methods of susceptibility for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacter spp. isolates
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Published in |
Brazilian Journal of Microbiology, May 2017
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DOI | 10.1016/j.bjm.2017.01.008 |
Pubmed ID | |
Authors |
Daniele Zendrini Rechenchoski, Angélica Marim Lopes Dambrozio, Ana Carolina Polano Vivan, Paulo Alfonso Schuroff, Tatiane das Neves Burgos, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero |
Abstract |
The production of KPC (Klebsiella pneumoniae carbapenemase) is the major mechanism of resistance to carbapenem agents in enterobacterias. In this context, forty KPC-producing Enterobacter spp. clinical isolates were studied. It was evaluated the activity of antimicrobial agents: polymyxin B, tigecycline, ertapenem, imipenem and meropenem, and was performed a comparison of the methodologies used to determine the susceptibility: broth microdilution, Etest(®) (bioMérieux), Vitek 2(®) automated system (bioMérieux) and disc diffusion. It was calculated the minimum inhibitory concentration (MIC) for each antimicrobial and polymyxin B showed the lowest concentrations for broth microdilution. Errors also were calculated among the techniques, tigecycline and ertapenem were the antibiotics with the largest and the lower number of discrepancies, respectively. Moreover, Vitek 2(®) automated system was the method most similar compared to the broth microdilution. Therefore, is important to evaluate the performance of new methods in comparison to the reference method, broth microdilution. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Australia | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 91 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 15 | 16% |
Researcher | 13 | 14% |
Student > Bachelor | 13 | 14% |
Student > Ph. D. Student | 9 | 10% |
Student > Doctoral Student | 3 | 3% |
Other | 9 | 10% |
Unknown | 29 | 32% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 11 | 12% |
Biochemistry, Genetics and Molecular Biology | 10 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 9 | 10% |
Agricultural and Biological Sciences | 8 | 9% |
Immunology and Microbiology | 6 | 7% |
Other | 15 | 16% |
Unknown | 32 | 35% |