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Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil

Overview of attention for article published in Brazilian Journal of Microbiology, February 2018
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Title
Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil
Published in
Brazilian Journal of Microbiology, February 2018
DOI 10.1016/j.bjm.2018.01.003
Pubmed ID
Authors

Joylson de Jesus Pereira, Natasha Baumworcel, Júlia Monassa Fioretti, Cinthya Fonseca Domingues, Laís Fernandes de Moraes, Robson dos Santos Souza Marinho, Maria Clara Rodrigues Vieira, Ana Maria Viana Pinto, Tatiana Xavier de Castro

Abstract

The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis along with most of the prototypes available in GenBank database and an amino acid analysis were performed. Phylogenetic analysis based on both conserved and variable region revealed two clusters with an aLTR value of 1.00 and 0.98 respectively and the variants from this study belong to feline calicivirus genogroup I. No association between geographical distribution and/or clinical signs and clustering in phylogenetic tree was observed. The variants circulating in public short-term animal shelter demonstrated a high variability because of the relatively rapid turnover of carrier cats constantly introduced of multiple viruses into this location over time.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 17%
Student > Bachelor 5 14%
Student > Doctoral Student 3 8%
Researcher 3 8%
Other 1 3%
Other 4 11%
Unknown 14 39%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 13 36%
Social Sciences 3 8%
Biochemistry, Genetics and Molecular Biology 2 6%
Agricultural and Biological Sciences 2 6%
Unspecified 1 3%
Other 2 6%
Unknown 13 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2018.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from Brazilian Journal of Microbiology
#824
of 1,377 outputs
Outputs of similar age
#253,163
of 344,055 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#16
of 33 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,055 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.