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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Screening of medium constituents for clavulanic acid production by Streptomyces clavuligerus
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, March 2018
|
| DOI | 10.1016/j.bjm.2018.01.006 |
| Pubmed ID | |
| Authors |
Kaio César da Silva Rodrigues, Arianne Tairyne de Souza, Alberto Colli Badino, Danielle Biscaro Pedrolli, Marcel Otavio Cerri |
| Abstract |
Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0.0-3.20gL-1), threonine (0.0-1.44gL-1), ornithine (0.0-4.08gL-1), and glutamate (0.0-8.16gL-1), according to a two-level central composite rotatable design. A medium containing ferrous sulfate yielded CA production of 437mgL-1, while a formulation without this salt produced only 41mgL-1of CA. This substantial difference suggested that Fe2+is important for CA biosynthesis. The experimental design showed that glutamate and ornithine negatively influenced CA production while arginine and threonine had no influence. The soybean protein isolate provided sufficient C5 precursor for CA biosynthesis, so that supplementation was unnecessary. Screening of medium components, together with experimental design tools, could be a valuable way of enhancing CA titers and reducing the process costs. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 4 | 15% |
| Student > Bachelor | 4 | 15% |
| Other | 3 | 12% |
| Researcher | 3 | 12% |
| Student > Ph. D. Student | 2 | 8% |
| Other | 6 | 23% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 19% |
| Engineering | 4 | 15% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Chemical Engineering | 3 | 12% |
| Social Sciences | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 6 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2018.
All research outputs
#16,053,755
of 25,382,440 outputs
Outputs from Brazilian Journal of Microbiology
#478
of 1,377 outputs
Outputs of similar age
#203,494
of 351,767 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#15
of 38 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,767 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.