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Evaluación de la espectrometría de masas: MALDI-TOF MS para la identificación rápida y confiable de levaduras

Overview of attention for article published in Revista Argentina de Microbiología, April 2015
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Title
Evaluación de la espectrometría de masas: MALDI-TOF MS para la identificación rápida y confiable de levaduras
Published in
Revista Argentina de Microbiología, April 2015
DOI 10.1016/j.ram.2015.02.004
Pubmed ID
Authors

María S. Relloso, Jimena Nievas, Santiago Fares Taie, Victoria Farquharson, María T. Mujica, Vanesa Romano, Mariela S. Zarate, Jorgelina Smayevsky

Abstract

The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 115 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 <1%
Unknown 114 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 29 25%
Student > Master 15 13%
Other 8 7%
Student > Postgraduate 6 5%
Student > Doctoral Student 5 4%
Other 9 8%
Unknown 43 37%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 15%
Medicine and Dentistry 14 12%
Agricultural and Biological Sciences 12 10%
Immunology and Microbiology 5 4%
Chemistry 5 4%
Other 17 15%
Unknown 45 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 April 2015.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Revista Argentina de Microbiología
#231
of 327 outputs
Outputs of similar age
#240,279
of 279,242 outputs
Outputs of similar age from Revista Argentina de Microbiología
#3
of 4 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 327 research outputs from this source. They receive a mean Attention Score of 1.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,242 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one.