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Evaluation and comparison of serological methods for the detection of bovine neosporosis in Argentina

Overview of attention for article published in Revista Argentina de Microbiología, September 2015
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Title
Evaluation and comparison of serological methods for the detection of bovine neosporosis in Argentina
Published in
Revista Argentina de Microbiología, September 2015
DOI 10.1016/j.ram.2015.07.002
Pubmed ID
Authors

Lucía M. Campero, Lieselotte Minke, Gastón Moré, Magdalena Rambeaud, Diana Bacigalupe, Dadin P. Moore, Yanina Hecker, Carlos M. Campero, Gereon Schares, María C. Venturini

Abstract

Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. The accurate diagnosis of neosporosis is essential for management and control measures. The aims of this study were: i) to evaluate the performance of an in-house enzyme-linked immunosorbent assay based on the 38kDa native antigen (p38-ELISA) to diagnose bovine neosporosis in Argentina using a well- characterized local sera panel from experimentally infected and naturally exposed cattle and ii) to compare the diagnostic performance and agreement of three N. caninum serological tests: p38-ELISA, indirect fluorescence antibody test (IFAT) and immunoblotting (IB) using the same sera panel. Serum samples testing either positive or negative by IFAT and IB were considered "Relative Standards of Comparison" (RSC) and used for p38-ELISA evaluation. Receiver operating characteristics analysis revealed that p38-ELISA was highly accurate (area under the curve= 0.982) according to RSC with a cut-off index of 0.0905. Relative sensitivity and specificity of p38-ELISA were 97.8% and 99.5%, respectively and agreement between RSC and p38-ELISA was almost perfect (k= 0.97). The evaluation and performance comparison of serological tests were performed according to the definition of gold standard based on the decision of the "majority of tests". All tests displayed high sensitivity and specificity values (greater than 95%); and excellent agreement. This study describes the accurate performance of p38-ELISA evaluated locally and the highly accurate diagnostic performance of the studied tests for the detection of anti-N. caninum antibodies in cattle from Argentina.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 25%
Student > Master 2 10%
Unknown 13 65%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 5 25%
Nursing and Health Professions 1 5%
Materials Science 1 5%
Unknown 13 65%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2015.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Revista Argentina de Microbiología
#231
of 327 outputs
Outputs of similar age
#245,738
of 286,825 outputs
Outputs of similar age from Revista Argentina de Microbiología
#10
of 12 outputs
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So far Altmetric has tracked 327 research outputs from this source. They receive a mean Attention Score of 1.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.