Title |
Prevalence and antibiotic susceptibility of coagulase-negative Staphylococcus species from bovine subclinical mastitis in dairy herds in the central region of Argentina
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Published in |
Revista Argentina de Microbiología, February 2016
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DOI | 10.1016/j.ram.2015.12.001 |
Pubmed ID | |
Authors |
Claudia G. Raspanti, Cesar C. Bonetto, Claudina Vissio, Matías S. Pellegrino, Elina B. Reinoso, Silvana A. Dieser, Cristina I. Bogni, Alejandro J. Larriestra, Liliana M. Odierno |
Abstract |
Coagulase-negative staphylococci (CNS) are a common cause of bovine subclinical mastitis (SCM). The prevalence of CNS species causing SCM identified by genotyping varies among countries. Overall, the antimicrobial resistance in this group of organisms is increasing worldwide; however, little information exists about a CNS species resistant to antibiotics. The aim of the present study was to genotypically characterize CNS at species level and to determine the prevalence and antibiotic resistance profiles of CNS species isolated from bovine SCM in 51 dairy herds located in the central region of the province of Cordoba, Argentina. In this study, we identified 219 CNS isolates at species level by PCR-restriction fragment length polymorphism of the groELgene. Staphylococcus chromogenes (46.6%) and Staphylococcus haemolyticus (32%) were the most prevalent species. A minimum of three different CNS species were present in 41.2% of the herds. S. chromogenes was isolated from most of the herds (86.3%), whereas S. haemolyticus was isolated from 66.7% of them. The broth microdilution method was used to test in vitro antimicrobial susceptibility. Resistance to a single compound or two related compounds was expressed in 43.8% of the isolates. S. chromogenes and S. haemolyticus showed a very high proportion of isolates resistant to penicillin. Resistance to two or more non-related antimicrobials was found in 30.6% of all CNS. S. haemolyticus exhibited a higher frequency of resistance to two or more non-related antimicrobials than S. chromogenes. |
X Demographics
Geographical breakdown
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 18% |
Student > Master | 5 | 15% |
Professor | 3 | 9% |
Student > Bachelor | 2 | 6% |
Researcher | 2 | 6% |
Other | 6 | 18% |
Unknown | 10 | 29% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 21% |
Veterinary Science and Veterinary Medicine | 6 | 18% |
Immunology and Microbiology | 6 | 18% |
Biochemistry, Genetics and Molecular Biology | 4 | 12% |
Nursing and Health Professions | 1 | 3% |
Other | 4 | 12% |
Unknown | 6 | 18% |