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Investigation on the effects of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa

Overview of attention for article published in Anais da Academia Brasileira de Ciências, May 2015
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Title
Investigation on the effects of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
Published in
Anais da Academia Brasileira de Ciências, May 2015
DOI 10.1590/0001-3765201520140286
Pubmed ID
Authors

Jaquelini Luber, Marcel J Palmieri, Carolina M Botelho, Daniel Rinaldo, Larissa F Andrade-Vieira

Abstract

Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa ("purple") - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than that of the infusions of the varieties Paluma and Pedro Sato. It was observed that seed germination and root growth in L. sativa exposed to infusions decreased with increasing infusion concentration, regardless of the tested cultivar. For the mitotic index, no statistical differences were observed. On the other hand, a significant increase in the frequency of cell cycle alterations was verified, especially for the highest concentrations tested. The cytogenotoxic effect was significant. Therefore, guava should not be used indiscriminately in popular medicine.

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Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 26%
Student > Master 5 16%
Student > Postgraduate 4 13%
Professor 3 10%
Student > Doctoral Student 2 6%
Other 3 10%
Unknown 6 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 32%
Chemistry 4 13%
Pharmacology, Toxicology and Pharmaceutical Science 3 10%
Environmental Science 3 10%
Biochemistry, Genetics and Molecular Biology 1 3%
Other 4 13%
Unknown 6 19%