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Mendeley readers
| Title |
Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
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|---|---|
| Published in |
Anais da Academia Brasileira de Ciências, May 2015
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| DOI | 10.1590/0001-3765201520140399 |
| Pubmed ID | |
| Authors |
Raniere M Moura, Arthur A Melo, Rômulo F Carneiro, Cícera R F Rodrigues, Plínio Delatorre, Kyria S Nascimento, Silvana Saker-Sampaio, Celso S Nagano, Benildo S Cavada, Alexandre H Sampaio |
| Abstract |
Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 13% |
| Professor | 4 | 13% |
| Student > Bachelor | 3 | 10% |
| Researcher | 3 | 10% |
| Student > Master | 3 | 10% |
| Other | 3 | 10% |
| Unknown | 10 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 30% |
| Agricultural and Biological Sciences | 3 | 10% |
| Chemistry | 2 | 7% |
| Computer Science | 1 | 3% |
| Materials Science | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 13 | 43% |