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X Demographics
Mendeley readers
| Title |
The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
|
|---|---|
| Published in |
Anais da Academia Brasileira de Ciências, September 2017
|
| DOI | 10.1590/0001-3765201720160825 |
| Pubmed ID | |
| Authors |
Arturo B Martinelli, Cecilia Waichert, Diego N Barbosa, Valéria Fagundes, Celso O Azevedo |
| Abstract |
Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 23% |
| Student > Ph. D. Student | 5 | 23% |
| Student > Master | 3 | 14% |
| Professor | 3 | 14% |
| Librarian | 2 | 9% |
| Other | 3 | 14% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 59% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Immunology and Microbiology | 2 | 9% |
| Computer Science | 1 | 5% |
| Earth and Planetary Sciences | 1 | 5% |
| Other | 2 | 9% |
| Unknown | 1 | 5% |