Title |
Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages
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Published in |
Revista da Sociedade Brasileira de Medicina Tropical, June 2016
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DOI | 10.1590/0037-8682-0190-2015 |
Pubmed ID | |
Authors |
Emanuella Francisco Fajardo, Marlene Cabrine-Santos, Keila Adriana Magalhães Ferreira, Eliane Lages-Silva, Luis Eduardo Ramírez, André Luiz Pedrosa |
Abstract |
This work shows that 3% (v/v) human urine (HU) in semisolid Liver Infusion Tryptose (SSL) medium favors the growth of Trypanosoma cruzi and T. rangeli. Parasites were plated as individual or mixed strains on SSL medium and on SSL medium with 3% human urine (SSL-HU). Isolate DNA was analyzed using polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE). SSL-HU medium improved clone isolation. PCR revealed that T. cruzi strains predominate on mixed-strain plates. PFGE confirmed that isolated parasites share the same molecular karyotype as parental cell lines. SSL-HU medium constitutes a novel tool for obtaining T. cruzi and T. rangeli clonal lineages. |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 2 | 13% |
Professor > Associate Professor | 2 | 13% |
Student > Master | 2 | 13% |
Other | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 2 | 13% |
Unknown | 5 | 33% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 4 | 27% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Agricultural and Biological Sciences | 1 | 7% |
Unknown | 6 | 40% |