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Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages

Overview of attention for article published in Revista da Sociedade Brasileira de Medicina Tropical, June 2016
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Title
Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages
Published in
Revista da Sociedade Brasileira de Medicina Tropical, June 2016
DOI 10.1590/0037-8682-0190-2015
Pubmed ID
Authors

Emanuella Francisco Fajardo, Marlene Cabrine-Santos, Keila Adriana Magalhães Ferreira, Eliane Lages-Silva, Luis Eduardo Ramírez, André Luiz Pedrosa

Abstract

This work shows that 3% (v/v) human urine (HU) in semisolid Liver Infusion Tryptose (SSL) medium favors the growth of Trypanosoma cruzi and T. rangeli. Parasites were plated as individual or mixed strains on SSL medium and on SSL medium with 3% human urine (SSL-HU). Isolate DNA was analyzed using polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE). SSL-HU medium improved clone isolation. PCR revealed that T. cruzi strains predominate on mixed-strain plates. PFGE confirmed that isolated parasites share the same molecular karyotype as parental cell lines. SSL-HU medium constitutes a novel tool for obtaining T. cruzi and T. rangeli clonal lineages.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 2 13%
Professor > Associate Professor 2 13%
Student > Master 2 13%
Other 1 7%
Student > Bachelor 1 7%
Other 2 13%
Unknown 5 33%
Readers by discipline Count As %
Immunology and Microbiology 4 27%
Pharmacology, Toxicology and Pharmaceutical Science 2 13%
Biochemistry, Genetics and Molecular Biology 2 13%
Agricultural and Biological Sciences 1 7%
Unknown 6 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2016.
All research outputs
#22,759,802
of 25,374,647 outputs
Outputs from Revista da Sociedade Brasileira de Medicina Tropical
#953
of 1,193 outputs
Outputs of similar age
#309,352
of 353,662 outputs
Outputs of similar age from Revista da Sociedade Brasileira de Medicina Tropical
#13
of 13 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,193 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,662 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.