Title |
Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran
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Published in |
Revista da Sociedade Brasileira de Medicina Tropical, August 2015
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DOI | 10.1590/0037-8682-0315-2014 |
Pubmed ID | |
Authors |
Khosrow Zamani, Amir Emami, Abdollah Bazargani, Afagh Moattari |
Abstract |
INTRODUCTIONThe aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates. Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The blaCTX-M cluster was identified using the RFLP method, and its genotype was sequenced. From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The blaCTX-M gene was identical to CTX-M-15. A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 5 | 25% |
Student > Postgraduate | 3 | 15% |
Professor > Associate Professor | 2 | 10% |
Student > Ph. D. Student | 2 | 10% |
Lecturer | 1 | 5% |
Other | 3 | 15% |
Unknown | 4 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Immunology and Microbiology | 4 | 20% |
Agricultural and Biological Sciences | 4 | 20% |
Medicine and Dentistry | 2 | 10% |
Unspecified | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 15% |