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Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR

Overview of attention for article published in Revista da Sociedade Brasileira de Medicina Tropical, August 2018
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Title
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR
Published in
Revista da Sociedade Brasileira de Medicina Tropical, August 2018
DOI 10.1590/0037-8682-0352-2017
Pubmed ID
Authors

Maristela Oliveira Lara, Thabata Coaglio Lucas, Evanguedes kalapothakis, Ronaldo Luis Thomasini, Carla Jorge Machado

Abstract

Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 17%
Lecturer 1 6%
Student > Doctoral Student 1 6%
Student > Ph. D. Student 1 6%
Student > Master 1 6%
Other 2 11%
Unknown 9 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 11%
Engineering 2 11%
Immunology and Microbiology 2 11%
Agricultural and Biological Sciences 1 6%
Chemistry 1 6%
Other 1 6%
Unknown 9 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 August 2018.
All research outputs
#20,663,600
of 25,385,509 outputs
Outputs from Revista da Sociedade Brasileira de Medicina Tropical
#740
of 1,193 outputs
Outputs of similar age
#265,424
of 341,886 outputs
Outputs of similar age from Revista da Sociedade Brasileira de Medicina Tropical
#7
of 16 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,193 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,886 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.