Title |
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR
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Published in |
Revista da Sociedade Brasileira de Medicina Tropical, August 2018
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DOI | 10.1590/0037-8682-0352-2017 |
Pubmed ID | |
Authors |
Maristela Oliveira Lara, Thabata Coaglio Lucas, Evanguedes kalapothakis, Ronaldo Luis Thomasini, Carla Jorge Machado |
Abstract |
Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 17% |
Lecturer | 1 | 6% |
Student > Doctoral Student | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Student > Master | 1 | 6% |
Other | 2 | 11% |
Unknown | 9 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 11% |
Engineering | 2 | 11% |
Immunology and Microbiology | 2 | 11% |
Agricultural and Biological Sciences | 1 | 6% |
Chemistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 9 | 50% |