Title |
Whole-genome sequences of influenza A(H3N2) viruses isolated from Brazilian patients with mild illness during the 2014 season
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Published in |
Memórias do Instituto Oswaldo Cruz, February 2015
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DOI | 10.1590/0074-02760140412 |
Pubmed ID | |
Authors |
Paola Cristina Resende, Fernando Couto Motta/+, Priscila Silva Born, Milene Miranda, Marilda M Siqueira |
Abstract |
The influenza A(H3N2) virus has circulated worldwide for almost five decades and is the dominant subtype in most seasonal influenza epidemics, as occurred in the 2014 season in South America. In this study we evaluate five whole genome sequences of influenza A(H3N2) viruses detected in patients with mild illness collected from January-March 2014. To sequence the genomes, a new generation sequencing (NGS) protocol was performed using the Ion Torrent PGM platform. In addition to analysing the common genes, haemagglutinin, neuraminidase and matrix, our work also comprised internal genes. This was the first report of a whole genome analysis with Brazilian influenza A(H3N2) samples. Considerable amino acid variability was encountered in all gene segments, demonstrating the importance of studying the internal genes. NGS of whole genomes in this study will facilitate deeper virus characterisation, contributing to the improvement of influenza strain surveillance in Brazil. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 2 | 5% |
Unknown | 41 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 16 | 37% |
Student > Ph. D. Student | 8 | 19% |
Student > Master | 5 | 12% |
Student > Bachelor | 4 | 9% |
Other | 2 | 5% |
Other | 2 | 5% |
Unknown | 6 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 12 | 28% |
Biochemistry, Genetics and Molecular Biology | 9 | 21% |
Medicine and Dentistry | 3 | 7% |
Immunology and Microbiology | 2 | 5% |
Nursing and Health Professions | 1 | 2% |
Other | 6 | 14% |
Unknown | 10 | 23% |