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Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, May 2015
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Title
Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes
Published in
Memórias do Instituto Oswaldo Cruz, May 2015
DOI 10.1590/0074-02760150024
Pubmed ID
Authors

S Andrea Moreno, Mayerly Nava

Abstract

Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisation of the glycolytic enzymes in T. evansi, which is a alike to that of the bloodstream form (BSF) of T. b. brucei: (i) fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hexokinase, phosphofructokinase, glucose-6-phosphate isomerase, phosphoglycerate kinase, triosephosphate isomerase (glycolytic enzymes) and glycerol-3-phosphate dehydrogenase (a glycolysis-auxiliary enzyme) in glycosomes, (ii) enolase, phosphoglycerate mutase, pyruvate kinase (glycolytic enzymes) and a GAPDH isoenzyme in the cytosol, (iii) malate dehydrogenase in cytosol and (iv) glucose-6-phosphate dehydrogenase in both glycosomes and the cytosol. Specific enzymatic activities also suggest that T. evansi is alike to the BSF of T. b. brucei in glycolytic flux, which is much faster than the pentose phosphate pathway flux, and in the involvement of cytosolic GAPDH in the NAD+/NADH balance. These similarities were expected based on the close phylogenetic relationship of both parasites.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 14%
Student > Postgraduate 2 9%
Student > Master 2 9%
Student > Bachelor 2 9%
Student > Doctoral Student 1 5%
Other 6 27%
Unknown 6 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 23%
Biochemistry, Genetics and Molecular Biology 2 9%
Veterinary Science and Veterinary Medicine 2 9%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Unspecified 1 5%
Other 4 18%
Unknown 7 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2015.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,299
of 1,502 outputs
Outputs of similar age
#238,565
of 279,492 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#19
of 23 outputs
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So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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