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Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, September 2016
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Title
Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
Published in
Memórias do Instituto Oswaldo Cruz, September 2016
DOI 10.1590/0074-02760160048
Pubmed ID
Authors

Juliana Failde Gallo, Juliana Maira Watanabe Pinhata, Erica Chimara, Maria Gisele Gonçalves, Lucila Okuyama Fukasawa, Rosangela Siqueira de Oliveira

Abstract

Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 20%
Student > Ph. D. Student 3 15%
Student > Bachelor 2 10%
Student > Postgraduate 2 10%
Professor > Associate Professor 1 5%
Other 0 0%
Unknown 8 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 25%
Pharmacology, Toxicology and Pharmaceutical Science 2 10%
Medicine and Dentistry 2 10%
Agricultural and Biological Sciences 1 5%
Veterinary Science and Veterinary Medicine 1 5%
Other 0 0%
Unknown 9 45%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2016.
All research outputs
#22,760,732
of 25,377,790 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,299
of 1,502 outputs
Outputs of similar age
#308,504
of 348,375 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#14
of 18 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.