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Experimental infection of Phlebotomus perniciosus by bioluminescent Leishmania infantum using murine model and artificial feeder

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, July 2016
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Title
Experimental infection of Phlebotomus perniciosus by bioluminescent Leishmania infantum using murine model and artificial feeder
Published in
Memórias do Instituto Oswaldo Cruz, July 2016
DOI 10.1590/0074-02760160100
Pubmed ID
Authors

Arnaud Cannet, Mohammad Akhoundi, Gregory Michel, Pierre Marty, Pascal Delaunay

Abstract

Leishmaniasis is a vector-borne disease that is transmitted by sandflies and caused by obligate intracellular protozoa of the genus Leishmania. In the present study, we carried out a screening on the experimental infection of Phlebotomus pernioucus by bioluminescent Leishmania infantum using murine model and artificial feeder. We developed a real-time polymerase chain reaction (RT-PCR)-based method to determine individually the number of Leishmania promastigotes fed by infected flies. Among 1840 new emerged female sand flies, 428 were fed on the infected mice. After their death, they were analysed individually by RT-PCR. Our results demonstrated just a single Leishmania positive female at sixth day post meal. A total of 1070 female sand flies were exposed in contact with artificial feeder containing the human blood with two different quantities of Leishmania parasites: 2.106/mL and 1.107/mL. A blood meal including 1.107/mL LUC-promastigotes was proposed to 270 females and 75 (28%) flies were engorged. Among them, 44 (59%) were positive by RT-PCR analysis, with a relative average of 50551 Leishmania parasites. In case of blood feeding of females with 2.106/mL promastigotes, 57 out of 800 (7%) females succeed to feed from artificial feeder which 22 (39%) were positive with a relative average of 6487 parasites.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 26%
Researcher 4 17%
Student > Ph. D. Student 4 17%
Student > Bachelor 2 9%
Other 1 4%
Other 2 9%
Unknown 4 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 22%
Agricultural and Biological Sciences 5 22%
Immunology and Microbiology 4 17%
Veterinary Science and Veterinary Medicine 2 9%
Nursing and Health Professions 1 4%
Other 1 4%
Unknown 5 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 July 2016.
All research outputs
#22,760,732
of 25,377,790 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,299
of 1,502 outputs
Outputs of similar age
#335,123
of 377,580 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#18
of 20 outputs
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So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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