Title |
First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil
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Published in |
Memórias do Instituto Oswaldo Cruz, January 2017
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DOI | 10.1590/0074-02760160283 |
Pubmed ID | |
Authors |
Thais de Araujo-Pereira, Daniela de Pita-Pereira, Mariana Côrtes Boité, Myllena Melo, Taiana Amancio da Costa-Rego, Andressa Alencastre Fuzari, Reginaldo Peçanha Brazil, Constança Britto |
Abstract |
Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subgenus in 12 out of 173 sandflies captured in the municipality of Rio Branco, Acre state, revealing a positivity of 6.94%. By sequencing the Leishmania 234 bp-hsp70 amplified products from positive samples, infection by L. (V.) braziliensis was confirmed in five sandflies: one Evandromyia saulensis, three Trichophoromyia auraensis and one Pressatia sp. The finding of L. (Viannia) DNA in two Ev. saulensis corresponds to the first record of possible infection associated with this sandfly. Moreover, our study reveals for the first time in Brazil, Th. auraensis and Pressatia sp. infected by L. (Viannia) parasites. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 2% |
Unknown | 50 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 16 | 31% |
Student > Bachelor | 8 | 16% |
Researcher | 6 | 12% |
Student > Postgraduate | 3 | 6% |
Student > Ph. D. Student | 2 | 4% |
Other | 2 | 4% |
Unknown | 14 | 27% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 11 | 22% |
Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Medicine and Dentistry | 8 | 16% |
Veterinary Science and Veterinary Medicine | 3 | 6% |
Immunology and Microbiology | 2 | 4% |
Other | 3 | 6% |
Unknown | 16 | 31% |