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An evaluation of false-positive rifampicin resistance on the Xpert MTB/RIF

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, November 2017
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Title
An evaluation of false-positive rifampicin resistance on the Xpert MTB/RIF
Published in
Memórias do Instituto Oswaldo Cruz, November 2017
DOI 10.1590/0074-02760170051
Pubmed ID
Authors

Yeliz Tanriverdi Cayci, Kemal Bilgin, Ahmet Yilmaz Coban, Asuman Birinci, Belma Durupınar

Abstract

Mycobacterium tuberculosis (MTB) is one of the most significant causes of mortality and morbidity. Early diagnose is important especially in multiple drug resistant tuberculosis to avoid transmission. Traditional techniques requires at least one to three weeks for diagnosis of tuberculosis. Diagnostic delays with multiple drug resistant tuberculosis are associated with worse clinical outcomes and increased transmission The Xpert MTB/RIF assay is one of the new diagnostic device for the diagnosis of tuberculosis and rapid detection of rifampicin resistance. We assessed the performance of Xpert MTB/RIF assay for detecting rifampicin resistance using phenotypic drug susceptibility tests as automated BD MGIT 960. Total of 2136 specimens were included in the study. Xpert MTB/RIF testing was performed on samples, using version 4 cartridges, according to the manufacturer's recommendations. The MTBC culture and first-line phenotypic DST were performed in automated BD MGIT 960 (Becton & Dickinson, USA) according to the recommendations of the manufacturer. Agar proportion was used in the case of inconsistency for rifampicin resistance. Thirty-four samples (19 respiratory and 15 nonrespiratory samples) were determined as positive for M. tuberculosis complex by Xpert MTB/RIF (Cepheid GeneXpert® System, USA). Xpert MTB/RIF assay detected 4/34 (11.7%) specimens as rifampicin resistant. One of the rifampicin resistant isolates was determined susceptible in MGIT 960 automated system. This isolate was also tested with agar proportion method and found susceptible to rifampicin. The Xpert MTB/RIF assay can be used as first-line assay for the detection of M. tuberculosis. However, microbiologists must be aware of the limitations of the assay.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 65 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 18%
Researcher 11 17%
Student > Ph. D. Student 10 15%
Other 5 8%
Student > Bachelor 3 5%
Other 8 12%
Unknown 16 25%
Readers by discipline Count As %
Medicine and Dentistry 23 35%
Immunology and Microbiology 8 12%
Biochemistry, Genetics and Molecular Biology 4 6%
Agricultural and Biological Sciences 3 5%
Veterinary Science and Veterinary Medicine 2 3%
Other 6 9%
Unknown 19 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2017.
All research outputs
#15,173,117
of 25,382,440 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#913
of 1,502 outputs
Outputs of similar age
#177,585
of 340,752 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#7
of 27 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 340,752 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.