Title |
Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
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Published in |
Memórias do Instituto Oswaldo Cruz, November 2017
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DOI | 10.1590/0074-02760170105 |
Pubmed ID | |
Authors |
Anne Drumond Villela, Valnês S Rodrigues-Junior, Antônio Frederico Michel Pinto, Virgínia Carla de Almeida Falcão, Zilpa Adriana Sánchez-Quitian, Paula Eichler, Cristiano Valim Bizarro, Luiz Augusto Basso, Diógenes Santiago Santos |
Abstract |
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence. |
X Demographics
Geographical breakdown
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 4 | 24% |
Student > Ph. D. Student | 3 | 18% |
Researcher | 2 | 12% |
Student > Bachelor | 1 | 6% |
Unspecified | 1 | 6% |
Other | 2 | 12% |
Unknown | 4 | 24% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 41% |
Immunology and Microbiology | 4 | 24% |
Unspecified | 1 | 6% |
Unknown | 5 | 29% |