Title |
Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
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Published in |
Memórias do Instituto Oswaldo Cruz, November 2013
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DOI | 10.1590/0074-0276130099 |
Pubmed ID | |
Authors |
Carolina De Marco Veríssimo, Vinícius José Maschio, Ana Paula Folmer Correa, Adriano Brandelli, Marilise Brittes Rott |
Abstract |
Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 18% |
Student > Ph. D. Student | 5 | 15% |
Professor > Associate Professor | 4 | 12% |
Researcher | 4 | 12% |
Student > Bachelor | 3 | 9% |
Other | 7 | 21% |
Unknown | 5 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 32% |
Immunology and Microbiology | 8 | 24% |
Biochemistry, Genetics and Molecular Biology | 3 | 9% |
Medicine and Dentistry | 3 | 9% |
Chemistry | 2 | 6% |
Other | 2 | 6% |
Unknown | 5 | 15% |