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The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader…

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, November 2013
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Title
The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region
Published in
Memórias do Instituto Oswaldo Cruz, November 2013
DOI 10.1590/0074-0276130201
Pubmed ID
Authors

Lina Marcela Villa, Felipe Guhl, Daniel Zabala, Juan David Ramírez, Daniel Alfonso Urrea, Diana Carolina Hernández, Zulma Cucunubá, Marleny Montilla, Julio César Carranza, Karina Rueda, Jorge Eduardo Trujillo, Gustavo Adolfo Vallejo

Abstract

A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruzi I to distinguish TcIDOMfrom sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 1%
Unknown 66 99%

Demographic breakdown

Readers by professional status Count As %
Student > Master 14 21%
Student > Ph. D. Student 8 12%
Student > Bachelor 7 10%
Researcher 7 10%
Student > Doctoral Student 6 9%
Other 13 19%
Unknown 12 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 36%
Medicine and Dentistry 14 21%
Biochemistry, Genetics and Molecular Biology 10 15%
Veterinary Science and Veterinary Medicine 1 1%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Other 2 3%
Unknown 15 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 February 2015.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,299
of 1,502 outputs
Outputs of similar age
#200,368
of 226,646 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#25
of 32 outputs
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