Title |
Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms
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Published in |
Brazilian Journal of Medical and Biological Research, November 2013
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DOI | 10.1590/1414-431x20133028 |
Pubmed ID | |
Authors |
K.K. Sakane, C.J. Monteiro, W. Silva, A.R. Silva, P.M. Santos, K.F. Lima, K.C.M. Moraes |
Abstract |
Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Student > Postgraduate | 2 | 14% |
Student > Master | 1 | 7% |
Lecturer | 1 | 7% |
Other | 2 | 14% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Environmental Science | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Other | 3 | 21% |
Unknown | 4 | 29% |