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X Demographics
Mendeley readers
| Title |
Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls
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|---|---|
| Published in |
Sao Paulo Medical Journal, July 2015
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| DOI | 10.1590/1516-3180.2014.8251809 |
| Pubmed ID | |
| Authors |
Isabel Azevedo Carvalho, David Germano Gonçalves Schwarz, Pricila Aparecida Grasse Pietralonga, Ana Carolina Silva Faria, Isis Freitas Espechit Braga, Gabriel Domingos Carvalho, Fabrício Luciani Valente, João Paulo Machado, Lize Maciel Pinheiro Guimarães, Maria de Lourdes Abreu Ferrari, Abelardo Silva, Maria Aparecida Scatamburlo Moreira |
| Abstract |
Mycobacterium avium subsp. paratuberculosis (MAP) has attracted the interest of researchers because of similarities between paratuberculosis and Crohn's disease (CD). The aim of this study was to evaluate the frequency of MAP through cultures, histology and polymerase chain reaction (PCR) on intestinal biopsies from Brazilian CD patients. Quantitative real time PCR (qRT-PCR) was performed on positive samples. Analytical cross-sectional study with control group at two federal universities. Fresh samples were collected from 25 patients; five with CD, eight with ulcerative colitis (UC) and 12 controls with non-inflammatory bowel disease (nIBD). Formalin-fixed paraffin-embedded (FFPE) samples from 143 patients were also collected: 44 CD, 49 UC and 56 nIBD. None of the fresh samples was positive for MAP. Five FFPE samples (one CD, two UC and two nIBD) and three fresh samples (one in each group) were positive through IS900-PCR. qRT-PCR was performed on these eight samples. Among the FFPE samples, there were 192.12 copies/μl in the CD group, 72.28 copies/μl in UC and 81.43 copies/μl in nIBD. Among the fresh samples, there were 432.99 copies/μl, 167.92 copies/μl and 249.73 copies/μl in the CD, UC and nIBD groups, respectively. The highest bacterial load was in the CD group. This study does not provide evidence for a role of MAP in the etiology of CD, although MAP DNA was detected in all three patient groups. This is the first report of MAP presence in human intestinal biopsies in Brazil. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 19% |
| Student > Ph. D. Student | 4 | 13% |
| Researcher | 4 | 13% |
| Student > Doctoral Student | 3 | 10% |
| Student > Master | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 11 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 16% |
| Biochemistry, Genetics and Molecular Biology | 4 | 13% |
| Immunology and Microbiology | 4 | 13% |
| Agricultural and Biological Sciences | 3 | 10% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 12 | 39% |