Title |
ITS-2 sequences-based identification of Trichogramma species in South America
|
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Published in |
Brazilian Journal of Biology, November 2015
|
DOI | 10.1590/1519-6984.04614 |
Pubmed ID | |
Authors |
R. P. Almeida, R. Stouthamer |
Abstract |
ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 20% |
Student > Master | 4 | 20% |
Researcher | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Student > Bachelor | 1 | 5% |
Other | 5 | 25% |
Unknown | 3 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 65% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Unspecified | 1 | 5% |
Medicine and Dentistry | 1 | 5% |
Engineering | 1 | 5% |
Other | 0 | 0% |
Unknown | 3 | 15% |