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Attention Score in Context
| Title |
Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean
|
|---|---|
| Published in |
Genetics and Molecular Biology, March 2017
|
| DOI | 10.1590/1678-4685-gmb-2016-0052 |
| Pubmed ID | |
| Authors |
Alessandra Jordano Conforte, Fábia Guimarães-Dias, Anna Cristina Neves-Borges, Marta Bencke-Malato, Durvalina Felix-Whipps, Márcio Alves-Ferreira |
| Abstract |
Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene glycol (PEG) and salt stress treatments. After 24 h of air-dried and PEG treatments, the pGAL-2kb led to an increase in GUS expression in leaf and root samples when compared to the control samples. These results were corroborated by qPCR expression analysis of the uidA gene. The pGAL-1kb showed no difference in GUS activity between control and treated samples. The pGAL-2kb promoter was evaluated in transgenic soybean roots, leading to an increase in EGFP expression under air-dried treatment. Our data indicates that pGAL-2kb could be a useful tool in developing drought-tolerant cultivars by driving gene expression. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| Unknown | 48 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 22% |
| Student > Master | 7 | 14% |
| Researcher | 6 | 12% |
| Student > Doctoral Student | 4 | 8% |
| Other | 4 | 8% |
| Other | 10 | 20% |
| Unknown | 7 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 55% |
| Biochemistry, Genetics and Molecular Biology | 7 | 14% |
| Environmental Science | 1 | 2% |
| Psychology | 1 | 2% |
| Social Sciences | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 9 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2017.
All research outputs
#17,289,387
of 25,382,440 outputs
Outputs from Genetics and Molecular Biology
#408
of 772 outputs
Outputs of similar age
#206,551
of 323,059 outputs
Outputs of similar age from Genetics and Molecular Biology
#9
of 18 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 772 research outputs from this source. They receive a mean Attention Score of 4.8. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.