Title |
Multidrug resistance in tumour cells: characterisation of the multidrug resistant cell line K562-Lucena 1
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Published in |
Anais da Academia Brasileira de Ciências, March 2001
|
DOI | 10.1590/s0001-37652001000100007 |
Pubmed ID | |
Authors |
VIVIAN M. RUMJANEK, GILMA S. TRINDADE, KAREN WAGNER-SOUZA, MICHELE C. MELETTI-DE-OLIVEIRA, LUIS F. MARQUES-SANTOS, RAQUEL C. MAIA, MÁRCIA A. M. CAPELLA |
Abstract |
Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mechanisms have relied on the analysis of cancer cell lines that have been selected and present cross-reactivity to a broad range of anticancer agents. This work characterises a multidrug resistant cell line, originally selected for resistance to the Vinca alkaloid vincristine and derived from the human erythroleukaemia cell K562. This cell line, named Lucena 1, overexpresses P-glycoprotein and have its resistance reversed by the chemosensitisers verapamil, trifluoperazine and cyclosporins A, D and G. Furthermore, we demonstrated that methylene blue was capable of partially reversing the resistance in this cell line. On the contrary, the use of 5-fluorouracil increased the resistance of Lucena 1. In addition to chemotherapics, Lucena 1 cells were resistant to ultraviolet A radiation and hydrogen peroxide and failed to mobilise intracellular calcium when thapsigargin was used. Changes in the cytoskeleton of this cell line were also observed. |
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