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Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil

Overview of attention for article published in Revista do Instituto de Medicina Tropical de Sao Paulo, February 2013
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Title
Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil
Published in
Revista do Instituto de Medicina Tropical de Sao Paulo, February 2013
DOI 10.1590/s0036-46652013000100002
Pubmed ID
Authors

Sarah Desirée Barbosa Cavalcanti, Mônica Scarpelli Martinelli Vidal, Maria da Glória Teixeira de Sousa, Gilda Maria Barbaro Del Negro

Abstract

Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features.

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The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 14%
Unknown 6 86%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 14%
Unknown 6 86%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2013.
All research outputs
#20,653,708
of 25,371,288 outputs
Outputs from Revista do Instituto de Medicina Tropical de Sao Paulo
#565
of 785 outputs
Outputs of similar age
#229,843
of 291,204 outputs
Outputs of similar age from Revista do Instituto de Medicina Tropical de Sao Paulo
#10
of 15 outputs
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So far Altmetric has tracked 785 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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