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Mendeley readers
Attention Score in Context
| Title |
Footprints of a trypanosomatid RNA world: pre-small subunit rRNA processing by spliced leader addition trans-splicing
|
|---|---|
| Published in |
Memórias do Instituto Oswaldo Cruz, June 2012
|
| DOI | 10.1590/s0074-02762012000400013 |
| Pubmed ID | |
| Authors |
Mario Gustavo Mayer, Marcos Gonzaga dos Santos, Maria Fernanda Laranjeira da Silva, Lucile Maria Floeter-Winter |
| Abstract |
The addition of a capped mini-exon [spliced leader (SL)] through trans-splicing is essential for the maturation of RNA polymerase (pol) II-transcribed polycistronic pre-mRNAs in all members of the Trypanosomatidae family. This process is an inter-molecular splicing reaction that follows the same basic rules of cis-splicing reactions. In this study, we demonstrated that mini-exons were added to precursor ribosomal RNA (pre-rRNA) are transcribed by RNA pol I, including the 5' external transcribed spacer (ETS) region. Additionally, we detected the SL-5'ETS molecule using three distinct methods and located the acceptor site between two known 5'ETS rRNA processing sites (A' and A1) in four different trypanosomatids. Moreover, we detected a polyadenylated 5'ETS upstream of the trans-splicing acceptor site, which also occurs in pre-mRNA trans-splicing. After treatment with an indirect trans-splicing inhibitor (sinefungin), we observed SL-5'ETS decay. However, treatment with 5-fluorouracil (a precursor of RNA synthesis that inhibits the degradation of pre-rRNA) led to the accumulation of SL-5'ETS, suggesting that the molecule may play a role in rRNA degradation. The detection of trans-splicing in these molecules may indicate broad RNA-joining properties, regardless of the polymerase used for transcription. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 7 | 27% |
| Student > Ph. D. Student | 6 | 23% |
| Researcher | 4 | 15% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 35% |
| Agricultural and Biological Sciences | 8 | 31% |
| Chemistry | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Immunology and Microbiology | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 4 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2014.
All research outputs
#20,655,488
of 25,371,288 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,185
of 1,502 outputs
Outputs of similar age
#139,926
of 179,218 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#9
of 14 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
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We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.