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Molecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, June 2013
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Title
Molecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism
Published in
Memórias do Instituto Oswaldo Cruz, June 2013
DOI 10.1590/s0074-02762013000400019
Pubmed ID
Authors

Richard Atherton, Darlene Bhavnani, Manuel Calvopiña, Yosselin Vicuña, William Cevallos, Joseph Eisenberg

Abstract

The aim of this study was to determine the genetic diversity of Giardia duodenalis present in a human population living in a northern Ecuadorian rain forest. All Giardia positive samples (based on an ELISA assay) were analysed using a semi-nested polymerase chain reaction-restriction fragment length polymorphism assay that targets the glutamate dehydrogenase (gdh) gene; those amplified were subsequently genotyped using NlaIV and RsaI enzymes. The gdh gene was successfully amplified in 74 of 154 ELISA positive samples; 69 of the 74 samples were subsequently genotyped. Of these 69 samples, 42 (61%) were classified as assemblage B (26 as BIII and 16 as BIV), 22 (32%) as assemblage A (3 as AI and 19 as AII) and five (7%) as mixed AII and BIII types. In this study site we observe similar diversity in genotypes to other regions in Latin America, though in contrast to some previous studies, we found similar levels of diarrheal symptoms in those individuals infected with assemblage B compared with those infected with assemblage A.

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Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 18%
Student > Ph. D. Student 5 11%
Student > Bachelor 4 9%
Researcher 4 9%
Professor 3 7%
Other 7 16%
Unknown 14 31%
Readers by discipline Count As %
Medicine and Dentistry 7 16%
Agricultural and Biological Sciences 7 16%
Biochemistry, Genetics and Molecular Biology 3 7%
Nursing and Health Professions 2 4%
Veterinary Science and Veterinary Medicine 2 4%
Other 6 13%
Unknown 18 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 August 2013.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,185
of 1,502 outputs
Outputs of similar age
#156,684
of 206,480 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#10
of 13 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.