Title |
Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation1
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Published in |
Acta Cirúrgica Brasileira, May 2015
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DOI | 10.1590/s0102-865020150050000008 |
Pubmed ID | |
Authors |
Lucas Matos Fernandes, Alessandro Wasum Mariani, Israel Lopes de Medeiros, Marcos Naoyuki Samano, Luís Gustavo Abdalla, Aristides Tadeu Correia, Natália Aparecida Nepomuceno, Mauro Canzian, Paulo Manuel Pêgo-Fernandes |
Abstract |
To evaluate a new perfusate solution to be used for ex vivo lung perfusion. Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative. From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion: SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361). The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors. |
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Other | 4 | 10% |
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