Penile reconstruction using mesenchymal stem cells1

Marcus Laks, Luiz Gonzaga Freitas-Filho, Kleber Sayeg, Mila Torii Correia Leite, Luciana Cristina Teixeira

To compare the reconstruction of corpus cavernosum segments when seeded with mesenchymal stem cells and when stem cells are infused intravenously. Sixteen New Zealand rabbits were submitted to reconstruction of the corpus cavernosum and distributed in Group A - decellularized matrices, Group B - decellularized matrices seeded with mesenchymal stem cells Group C - decellularized matrices submitted to intravenous infusion of mesenchymal stem cells. The mesenchymal stem cells were obtained by bone marrow aspiration. The venous filling aspect of the distal end of the corpus cavernosum was evaluated and the specimens were submitted to histological analisis and to immunohistochemistry. Cavernosometry was done in one animal of each group Three animals on B and three animals on C presented full filling of distal end of the corpus cavernosum. No animals in A presented filling of the distal end of corpus cavernosum. At cavernosometry the animal on B attained 50 cmH2O, on C 110 cmH2O and on A 20 cmH2O. Trabeculae forming cavernous sinuses were found in groups B and C. The reconstruction of corpus cavernosum using descellularized matrices and mesenchymal stem cells, either by intravenous injection or directly seeded is possible, with growth of corpus cavernosum-like tissue.