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Attention Score in Context
| Title |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, December 2013
|
| DOI | 10.1590/s1517-83822013000400007 |
| Pubmed ID | |
| Authors |
Janaína T. de Faria, Pollyana F. Rocha, Attilio Converti, Flávia M.L. Passos, Luis A. Minim, Fábio C. Sampaio |
| Abstract |
The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment time. Such an approach allowed us to identify an optimal range of the independent variables within which the β-galactosidase activity was optimized. A maximum permeabilization of 2,816 mmol L(-1) oNP min(-1) g(-1) was obtained by treating cells with 75.0% v/v of ethanol at 20.0 °C for 15.0 min. The proposed methodology resulted to be effective and suited for K. lactis cells permeabilization at a lab-scale and promises to be of possible interest for future applications mainly in the food industry. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Thailand | 1 | 2% |
| Unknown | 40 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 15% |
| Student > Bachelor | 5 | 12% |
| Researcher | 5 | 12% |
| Student > Master | 4 | 10% |
| Professor > Associate Professor | 3 | 7% |
| Other | 6 | 15% |
| Unknown | 12 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 32% |
| Biochemistry, Genetics and Molecular Biology | 8 | 20% |
| Chemical Engineering | 2 | 5% |
| Unspecified | 2 | 5% |
| Engineering | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 12 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2015.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Brazilian Journal of Microbiology
#1,047
of 1,377 outputs
Outputs of similar age
#282,779
of 320,964 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#21
of 40 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.