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Optimization of zofimarin production by an endophytic fungus, Xylaria sp. Acra L38

Overview of attention for article published in Brazilian Journal of Microbiology, January 2014
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Title
Optimization of zofimarin production by an endophytic fungus, Xylaria sp. Acra L38
Published in
Brazilian Journal of Microbiology, January 2014
DOI 10.1590/s1517-83822014000100042
Pubmed ID
Authors

Jirapan Chaichanan, Suthep Wiyakrutta, Thunyarat Pongtharangkul, Duangnate Isarangkul, Vithaya Meevootisom

Abstract

To optimize the medium for high zofimarin production, sucrose maltose, glucose, tryptone and peptone were used in an orthogonal array design experiment, where the highest value of zofimarin produced was 25.6 μg/mL. This value was about 3 times higher than that obtained with Czapek yeast extract (CzYE) culture medium. A study with Plackett-Burman design showed that sucrose, maltose, glucose and NaNO3 were significant factors in zofimarin production. Further studies using central composite design (CCD) showed the significance of glucose and the interactions of these critical components affecting zofimarin production. Multiple regression analysis of the data yielded a poor fit as shown by the mismatch of the model with these variable factors. When a polynomial equation was applied, the maximum zofimarin production was predicted to be 201.9 μg/mL. Experimental verification yielded a much lower amount of zofimarin, at around 70 μg/mL. Reconsideration of the CCD data and repetition of some runs with high zofimarin production resulted in reproducible zofimarin yield at 79.7 μg/mL. Even though the amount was lower than the predicted value, the medium optimization study was considered to be quite successful as the yield increased to around 8 times that obtained with the original CzYE culture medium.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Egypt 1 3%
Unknown 33 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 26%
Student > Master 5 15%
Student > Doctoral Student 4 12%
Other 3 9%
Researcher 3 9%
Other 4 12%
Unknown 6 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 26%
Immunology and Microbiology 5 15%
Biochemistry, Genetics and Molecular Biology 4 12%
Environmental Science 3 9%
Chemistry 2 6%
Other 2 6%
Unknown 9 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2014.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Brazilian Journal of Microbiology
#1,047
of 1,377 outputs
Outputs of similar age
#280,467
of 319,271 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#21
of 40 outputs
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.