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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, June 2014
|
| DOI | 10.1590/s1517-83822014000200035 |
| Pubmed ID | |
| Authors |
Cristina P. Araújo, Ana Luiza A.R. Osório, Klaudia S.G. Jorge, Carlos A.N. Ramos, Antonio F. Souza Filho, Carlos E.S. Vidal, Agueda P.C. Vargas, Eliana Roxo, Adalgiza S. Rocha, Philip N. Suffys, Antônio A. Fonseca, Marcio R. Silva, José D. Barbosa Neto, Valíria D. Cerqueira, Flábio R. Araújo |
| Abstract |
Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Thailand | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 48 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 17% |
| Researcher | 7 | 15% |
| Student > Master | 6 | 13% |
| Other | 4 | 8% |
| Student > Bachelor | 4 | 8% |
| Other | 7 | 15% |
| Unknown | 12 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 11 | 23% |
| Agricultural and Biological Sciences | 9 | 19% |
| Medicine and Dentistry | 5 | 10% |
| Biochemistry, Genetics and Molecular Biology | 2 | 4% |
| Social Sciences | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 15 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2015.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Brazilian Journal of Microbiology
#593
of 1,377 outputs
Outputs of similar age
#144,714
of 240,964 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#6
of 25 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,964 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.