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Attention Score in Context
| Title |
Milk-deteriorating exoenzymes from Pseudomonas fluorescens 041 isolated from refrigerated raw milk
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, May 2015
|
| DOI | 10.1590/s1517-838246120130859 |
| Pubmed ID | |
| Authors |
Maurilio L. Martins, Uelinton M. Pinto, Katharina Riedel, Maria C.D. Vanetti |
| Abstract |
The practice of refrigerating raw milk at the farm has provided a selective advantage for psychrotrophic bacteria that produce heat-stable proteases and lipases causing severe quality problems to the dairy industry. In this work, a protease (AprX) and a lipase (LipM) produced by Pseudomonas fluorescens 041, a highly proteolytic and lipolytic strain isolated from raw milk obtained from a Brazilian farm, have been purified and characterized. Both enzymes were purified as recombinant proteins from Escherichia coli . The AprX metalloprotease exhibited activity in a broad temperature range, including refrigeration, with a maximum activity at 37 °C. It was active in a pH range of 4.0 to 9.0. This protease had maximum activity with the substrates casein and gelatin in the presence of Ca (+2) . The LipM lipase had a maximum activity at 25 °C and a broad pH optimum ranging from 7.0 to 10. It exhibited the highest activity, in the presence of Ca (+2) , on substrates with long-chain fatty acid residues. These results confirm the spoilage potential of strain 041 in milk due to, at least in part, these two enzymes. The work highlights the importance of studies of this kind with strains isolated in Brazil, which has a recent history on the implementation of the cold chain at the dairy farm. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 1% |
| Unknown | 66 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 14 | 21% |
| Student > Bachelor | 6 | 9% |
| Student > Ph. D. Student | 5 | 7% |
| Professor | 5 | 7% |
| Unspecified | 4 | 6% |
| Other | 13 | 19% |
| Unknown | 20 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 25% |
| Biochemistry, Genetics and Molecular Biology | 5 | 7% |
| Engineering | 5 | 7% |
| Unspecified | 4 | 6% |
| Business, Management and Accounting | 2 | 3% |
| Other | 12 | 18% |
| Unknown | 22 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2024.
All research outputs
#7,356,550
of 25,374,917 outputs
Outputs from Brazilian Journal of Microbiology
#154
of 1,377 outputs
Outputs of similar age
#81,990
of 278,918 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#4
of 45 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,918 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.