Title |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium
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Published in |
Brazilian Journal of Microbiology, June 2015
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DOI | 10.1590/s1517-838246120131276 |
Pubmed ID | |
Authors |
Santina Santisi, Simone Cappello, Maurizio Catalfamo, Giuseppe Mancini, Mehdi Hassanshahian, Lucrezia Genovese, Laura Giuliano, Michail M. Yakimov |
Abstract |
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Portugal | 1 | <1% |
Colombia | 1 | <1% |
Brazil | 1 | <1% |
United Kingdom | 1 | <1% |
Estonia | 1 | <1% |
Philippines | 1 | <1% |
Unknown | 145 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 26 | 17% |
Student > Ph. D. Student | 19 | 13% |
Researcher | 16 | 11% |
Student > Master | 15 | 10% |
Other | 8 | 5% |
Other | 25 | 17% |
Unknown | 42 | 28% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 30 | 20% |
Agricultural and Biological Sciences | 23 | 15% |
Environmental Science | 20 | 13% |
Immunology and Microbiology | 7 | 5% |
Chemistry | 7 | 5% |
Other | 18 | 12% |
Unknown | 46 | 30% |