Title |
Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites
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Published in |
Brazilian Journal of Microbiology, May 2015
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DOI | 10.1590/s1517-838246120140026 |
Pubmed ID | |
Authors |
Hatice Aysun Mercimek, Sadik Dincer, Gulcihan Guzeldag, Aysenur Ozsavli, Fatih Matyar, Afet Arkut, Fikret Kayis, Melis Sumengen Ozdenefe |
Abstract |
Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitro experiments . Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L (-1) TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL (-1) TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 7 | 21% |
Researcher | 6 | 18% |
Student > Master | 4 | 12% |
Student > Ph. D. Student | 3 | 9% |
Student > Doctoral Student | 2 | 6% |
Other | 5 | 15% |
Unknown | 6 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 18% |
Environmental Science | 5 | 15% |
Agricultural and Biological Sciences | 4 | 12% |
Chemistry | 4 | 12% |
Nursing and Health Professions | 1 | 3% |
Other | 4 | 12% |
Unknown | 9 | 27% |