Title |
Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh)
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Published in |
Brazilian Journal of Microbiology, June 2015
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DOI | 10.1590/s1517-838246220140208 |
Pubmed ID | |
Authors |
Liu Hongming, Lou Xu, Ge Zhaojian, Yang Fan, Chen Dingbin, Zhu Jianchun, Xu Jianhong, Li Shunpeng, Hong Qing |
Abstract |
The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L(-1) FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Researcher | 2 | 14% |
Student > Master | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 0 | 0% |
Unknown | 6 | 43% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 14% |
Environmental Science | 1 | 7% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Medicine and Dentistry | 1 | 7% |
Other | 1 | 7% |
Unknown | 7 | 50% |