Title |
GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium
|
---|---|
Published in |
Brazilian Journal of Microbiology, September 2015
|
DOI | 10.1590/s1517-838246320120226 |
Pubmed ID | |
Authors |
Qun Luo, Ying He, Deng-Yong Hou, Jian-Guo Zhang, Xian-Rong Shen |
Abstract |
To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1 gene in E. coli DH5α could enhance the function of diesel oil degradation by the bacterial consortium. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 3% |
Unknown | 30 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 16% |
Student > Master | 4 | 13% |
Student > Doctoral Student | 3 | 10% |
Student > Bachelor | 3 | 10% |
Librarian | 1 | 3% |
Other | 2 | 6% |
Unknown | 13 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 23% |
Environmental Science | 3 | 10% |
Agricultural and Biological Sciences | 3 | 10% |
Computer Science | 1 | 3% |
Social Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 15 | 48% |