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Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain

Overview of attention for article published in Brazilian Journal of Microbiology, September 2015
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Title
Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
Published in
Brazilian Journal of Microbiology, September 2015
DOI 10.1590/s1517-838246320131289
Pubmed ID
Authors

Jiang Li, Qiushi Hu, Yuquan Li, Yuan Xu

Abstract

An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg(2+), Mn(2+), K(+); Ca(2+), Na(+), Ba(2+), Zn(2+), Cu(2+), Co(2+), Fe(2+), Sr(2+) and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 4%
Unknown 27 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 14%
Student > Bachelor 4 14%
Student > Doctoral Student 3 11%
Professor 3 11%
Lecturer 2 7%
Other 7 25%
Unknown 5 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 32%
Biochemistry, Genetics and Molecular Biology 9 32%
Immunology and Microbiology 3 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Social Sciences 1 4%
Other 0 0%
Unknown 5 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 September 2015.
All research outputs
#20,660,571
of 25,377,790 outputs
Outputs from Brazilian Journal of Microbiology
#887
of 1,377 outputs
Outputs of similar age
#203,166
of 276,791 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#17
of 25 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.