Title |
Phylogenetic analysis and genotype distribution of Hepatitis B Virus (HBV) in Roraima, Brazil
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Published in |
Revista do Instituto de Medicina Tropical de Sao Paulo, July 2018
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DOI | 10.1590/s1678-9946201860035 |
Pubmed ID | |
Authors |
Débora Dinelly de Sousa, Claudiane Raquel de Sousa Silva, Wilson Pereira Lima, Jacqueline de Aguiar Barros, Ismael Alexandre da Silva Nascimento, Victor Costa de Souza, Felipe Gomes Naveca, Fabiana Granja |
Abstract |
Hepatitis B virus (HBV) infection is a serious global health problem. HBV has a high viral genetic diversity, with 10 genotypes recognized. In Brazil, the Roraima State is the third in the Northern region regarding the number of hepatitis B cases. On the other hand, few data on HBV genotyping and phylogenetic analysis are available. The purpose of this study is to characterize the HBV genotypes circulating in Roraima State. Of the 113 chronic hepatitis B patients enrolled in this study, 40 were HBV-DNA positive. A fragment of 280 bp (S gene) was amplified by PCR and submitted to nucleotide sequencing. A dataset containing the viral sequences obtained in this study, plus 130 obtained from GenBank was used for genotyping by phylogenetic analysis. The HBV subgenotype distribution found was A1 (62.5%), A2 (7.5%), D2, D3, D4 (2.5%), F2a (12.5%), and F3 (10%). We characterized the genotypes and subgenotypes of HBV circulating among patients in the State of Roraima. In addition, our study shows for the first time the HBV/F3 genotype circulating in Brazil. In conclusion, our findings showed a high diversity of HBV genotypes in Roraima, which is also found in other Brazilian geographical regions. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 4 | 16% |
Student > Bachelor | 3 | 12% |
Researcher | 3 | 12% |
Student > Postgraduate | 2 | 8% |
Student > Ph. D. Student | 2 | 8% |
Other | 3 | 12% |
Unknown | 8 | 32% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 4 | 16% |
Agricultural and Biological Sciences | 3 | 12% |
Environmental Science | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Other | 5 | 20% |
Unknown | 10 | 40% |