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Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures

Overview of attention for article published in Revista Brasileira de Parasitologia Veterinária, March 2013
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Title
Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
Published in
Revista Brasileira de Parasitologia Veterinária, March 2013
DOI 10.1590/s1984-29612013000100026
Pubmed ID
Authors

Fabio Ribeiro Braga, Jackson Victor de Araújo, Filippe Elias de Freitas Soares, Juliana Milani Araujo, Sebastião Rodrigo Ferreira, Alexandre de Oliveira Tavela, Wendeo Ferreira da Silveira, José Humberto de Queiroz

Abstract

The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L3) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722); group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water). The third-stage larvae (L3) were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05). The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungus D. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Professor 3 25%
Student > Doctoral Student 2 17%
Student > Master 2 17%
Student > Bachelor 1 8%
Researcher 1 8%
Other 0 0%
Unknown 3 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 2 17%
Environmental Science 2 17%
Agricultural and Biological Sciences 2 17%
Immunology and Microbiology 1 8%
Unknown 5 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2015.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from Revista Brasileira de Parasitologia Veterinária
#172
of 660 outputs
Outputs of similar age
#128,254
of 206,322 outputs
Outputs of similar age from Revista Brasileira de Parasitologia Veterinária
#2
of 21 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 660 research outputs from this source. They receive a mean Attention Score of 1.2. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 206,322 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.