Title |
Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
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Published in |
Revista Brasileira de Parasitologia Veterinária, March 2013
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DOI | 10.1590/s1984-29612013000100026 |
Pubmed ID | |
Authors |
Fabio Ribeiro Braga, Jackson Victor de Araújo, Filippe Elias de Freitas Soares, Juliana Milani Araujo, Sebastião Rodrigo Ferreira, Alexandre de Oliveira Tavela, Wendeo Ferreira da Silveira, José Humberto de Queiroz |
Abstract |
The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L3) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722); group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water). The third-stage larvae (L3) were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05). The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungus D. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 3 | 25% |
Student > Doctoral Student | 2 | 17% |
Student > Master | 2 | 17% |
Student > Bachelor | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 2 | 17% |
Environmental Science | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Immunology and Microbiology | 1 | 8% |
Unknown | 5 | 42% |