Title |
Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera
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Published in |
Revista Brasileira de Parasitologia Veterinária, August 2015
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DOI | 10.1590/s1984-29612015055 |
Pubmed ID | |
Authors |
Rômulo Silva de Oliveira, Rodrigo Casquero Cunha, Jonas Moraes-Filho, Relber Aguiar Gonçales, Ana de Souza Stori de Paula Lara, Luciana Farias da Costa de Avila, Marcelo Bahia Labruna, Fábio Pereira Leivas Leite |
Abstract |
The canine monocytic ehrlichiosis, caused by Ehrlichia canis, is endemic in several regions of Brazil. Some serological diagnostic techniques using immunodominant proteins of E. canis as antigens are available, but their specificities and sensitivities are questionable. Based on this, the objective of this study was to test the antigenic potential of the recombinant gp19 protein (rGP19) for subsequent use in diagnostic tests. The rGP19 expressed in the Escherichia coli strain BL21 (DE3) C41 was recognized in the sera from experimentally infected dogs using ELISA and Western blotting. Thus, it was possible to obtain a promising antigen with the ability to differentiate between E. canis-positive and -negative animals, even 1 week after infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 19% |
Student > Bachelor | 4 | 15% |
Student > Postgraduate | 4 | 15% |
Student > Master | 4 | 15% |
Professor | 2 | 7% |
Other | 3 | 11% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 8 | 30% |
Agricultural and Biological Sciences | 5 | 19% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Medicine and Dentistry | 2 | 7% |
Immunology and Microbiology | 1 | 4% |
Other | 2 | 7% |
Unknown | 7 | 26% |