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Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil

Overview of attention for article published in Revista Brasileira de Parasitologia Veterinária, August 2018
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Title
Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil
Published in
Revista Brasileira de Parasitologia Veterinária, August 2018
DOI 10.1590/s1984-296120180059
Pubmed ID
Authors

Marcio André Silva, Hilda Fátima Jesus Pena, Herbert Sousa Soares, Juliana Aizawa, Solange Oliveira, Bruna Farias Alves, Dênisson Silva Souza, Renata Pimentel Bandeira Melo, Solange Maria Gennari, Rinaldo Aparecido Mota, Jean Carlos Ramos Silva

Abstract

Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 5'3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype #13 from the striated heron isolate and Type BrIII genotype from a captive otter ( Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 21%
Student > Bachelor 5 15%
Researcher 5 15%
Other 3 9%
Student > Ph. D. Student 3 9%
Other 3 9%
Unknown 7 21%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 8 24%
Agricultural and Biological Sciences 8 24%
Biochemistry, Genetics and Molecular Biology 3 9%
Medicine and Dentistry 2 6%
Earth and Planetary Sciences 2 6%
Other 2 6%
Unknown 8 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2019.
All research outputs
#14,920,631
of 25,385,509 outputs
Outputs from Revista Brasileira de Parasitologia Veterinária
#80
of 660 outputs
Outputs of similar age
#176,518
of 344,376 outputs
Outputs of similar age from Revista Brasileira de Parasitologia Veterinária
#4
of 19 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 660 research outputs from this source. They receive a mean Attention Score of 1.2. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,376 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.